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Precision Oncology Resources: Webinars

Associate Professor,
UCSD

This webinar will discuss emerging biomarkers for thyroid cancer and non-small cell lung cancer, with a particular focus on detecting gene fusions such as RET.

Our speaker, Dr. Sandip Patel of UCSD, will provide an overview of biomarker-guided therapies for thyroid cancer and NSCLC and will discuss key emerging biomarkers for these indications along with their supporting evidence.

Dr. Patel will also discuss different biomarker testing approaches, practical considerations, and address challenges around fusion detection.

The webinar will highlight hybrid-capture based comprehensive genomic profiling as a preferred method, along with a background and overview of this approach.

Clinical Lab Support Coordinator, Molecular Diagnostics Laboratory,
Sentara Healthcare Laboratory Services

Associate Chief of Clinical Pathology and Medical Director Molecular and Microbiology Laboratories,
Baystate Health

This webinar will address how two molecular laboratories are implementing tertiary analysis software to improve their precision oncology workflows.

Solid tumor biomarker analysis by next generation sequencing (NGS) allows for better tissue stewardship and more efficient delivery of actionable results than other methodologies like single gene sequencing, but data analysis and interpretation remains a significant barrier to NGS adoption in community settings. In particular, the final tertiary analysis of NGS data is complicated by several factors, including variant-to-variant interactions, variant curation, and time to report.

Our first speaker, Dr. David Seidman of Sentara Healthcare Laboratory Serviceswill discuss how automated interpretation of NGS variants with Navify Mutation Profiler reduces the need for time-consuming and error-prone manual analysis. Software capable of automatically identifying, interpreting, and visualizing clinically relevant variant combinations promises to further decrease the manual review time necessary to account for relevant variant combinations. In a diagnostic setting, addressing variant-variant interplay in an automated fashion may also help prevent inaccurate reporting that can adversely affect the test subject.         

Our second speaker, Dr. Franklin Moore of Baystate Healthwill discuss his experiences integrating Navify Mutation Profiler into his lab's workflow, both during the evaluation phase and the clinical validation. Dr. Moore will present data on variant detection, annotation, interpretation, and tier classification compared to a manual pipeline method. He will also address, issues, challenges, and future directions.

Co-Director, Solid Tumor and Constitutional Disease Diagnostic Laboratory,
University of Navarra

This webinar will review how liquid biopsy can be considered as an alternative and non-invasive method to tissue biopsy for cancer molecular characterization.

Liquid biopsy enables the study of tumor-specific circulating components, including circulating tumor cells (CTCs), circulating cell-free tumor DNA (ctDNA) and RNA (ctRNA). The approach brings both great potential and new challenges to precision medicine.

This webinar will highlight two key aspects of successful implementation of liquid biopsy in the laboratory: proficiency testing and interlaboratory comparison. Additionally, our speaker, Dr. Gorka Alkorta-Aranburu of University of Navarra, will discuss specifics of the liquid biopsy methodology, including properties of cell-free nucleic acids and considerations for next-generation sequencing technology.

Learning objectives:

  • The benefits of liquid biopsy as a non-invasive method to better understand cancer development and treatment.
  • The advantages (and challenges) of an NGS approach to study circulating cell-free nucleic acids for clinical applications.

*This webinar will be pre-recorded.  You may submit questions for our speaker on the registration page.

VP of Research and Development, NeoGenomics

This webinar will provide a first-hand look at how a clinical lab evolved its tumor profiling workflow from a targeted panel approach toward comprehensive genomic profiling.  

Cancer is a highly complex disease and understanding the various alterations that together determine mechanisms of disease onset, progression, recurrence, and response to treatment is not trivial. These alterations, however, are not all found at the DNA level. Multimodal approaches can generate a profile of these various alterations, but this approach requires a fragmented and challenging workflow.

More recently, the field has started moving toward comprehensive genomic profiling (CGP), enabling a snapshot of the genetic makeup of the tumor at a specific timepoint. This approach provides insights beyond mutations at the nucleotide level that may provide a better understanding of the state of the disease and the various pathways that modulate its progression and response to its environment, including treatment.

In this webinar, Vincent Funari of NeoGenomics will discuss how his team has evolved its profiling approaches for both heme and solid tumors from targeted panels to more comprehensive genomic profiling.

Dr. Funari will highlight tools that enable a more streamlined path for adopting CGP, enabling operational efficiencies such as reduced overall cost while delivering high-quality data. He will also share additional insights his team gained into alterations that were previously missed due to limitations of current technologies.

Sponsored by

Pathologist,
Moffitt Cancer Center

This webinar will discuss how Moffitt Cancer Center has implemented a new capture-based application to accurately assess myeloid malignancies by detecting complex variants in challenging genes in a single experiment.  

Molecular profiling by next-generation sequencing (NGS) of myeloid tumors has become a routine part of disease management. One of the difficulties and limitations of NGS technology has historically been the inability to reliably detect mutations in certain GC-rich gene regions (such as the CEBPA gene) and insertions/deletions in genes such as FLT3, NPM1, and CALR. 

Many labs have circumvented these limitations by performing parallel orthogonal testing, which is redundant, costly, and inefficient. Furthermore, in late 2018, the US Food and Drug Administration approved a targeted therapy for FLT3-mutated acute myeloid leukemia, making accurate and reproducible mutation detection of paramount importance for guiding treatment.

In this webinar, Dr. Mohammad Hussaini of the Moffitt Cancer Center will discuss development of a comprehensive solution that captures 98 genes noted to be of importance in myeloid disease. In particular, he will describe:     

  • The process of evaluating and implementing this new capture-based NGS solution 
  • The accurate detection of challenging genes such as FLT3, CALR, and CEBPA 
  • The global analytical performance of this solution
Recent GenomeWebinars

Research Technician, Jan Ellenberg Lab, European Molecular Biology Laboratory

Research Technician,  Jan Ellenberg Lab, European Molecular Biology Laboratory

Fluorescent proteins or self-labeling tags are invaluable tools for studying protein dynamics in living cells using fluorescence microscopy. However, quantitative imaging requires physiological levels of expression of the target protein of interest (POI), especially when stoichiometric interactions of the POI need to be investigated.

CRISPR has enabled researchers to tag virtually any target gene of interest, resulting in physiological levels of expression of the corresponding POI. However, the generation and selection of cellular clones bearing the correct edit — that is, the expected number of tagged alleles and an absence of extra integrations — requires quantitative assessment of the tag copy number.

This webinar will describe the use of dPCR as a quick method for quantitation of green fluorescent protein copy number in CRISPR-edited HeLa cells. The discussion will also include an introduction to dPCR working principles and post-acquisition data analysis methods.

Mon
Nov
18
1:00 pm2019
Sponsored by
Mission Bio

Simultaneous Single-Cell DNA and Protein Sequencing in AML Samples

Genome Webinar

Postdoctoral Research Fellow,
Memorial Sloan Kettering Cancer Center

This webinar will outline the results of a study that performed integrative single-cell genome and cell surface protein expression profiling of adult acute myeloid leukemia (AML) cases. 

Genomic studies of AML have identified mutations with varying allele frequencies, which suggests a step-wise acquisition of mutation in leukemogenesis. Bulk sequencing can provide prognostic information, but it cannot delineate clonal architecture at the single-cell level. Additionally, the complementation of immunophenotype with corresponding mutational analysis could elucidate important dynamics in the leukemic cell population, but has yet to be assayed in parallel.

In this webinar, Linde Miles of Memorial Sloan Kettering Cancer Center will share data from the study demonstrating that simultaneous single-cell sequencing of DNA and protein can identify populations of stem/progenitor cells with distinct clonal/mutational and protein expression profiles. She will also discuss how the method identified trends in protein expression when comparing multiple samples with similar mutations. 

Attendees of this webinar will learn about:

  • New DNA+protein capability from Mission Bio for simultaneous, targeted, single-cell DNA and protein sequencing
  • A novel 6-antibody cocktail in combination with a single-cell targeted DNA custom AML panel 
  • The relationship between mutational profile and cell surface protein expression in AML samples from six clinical research samples 
Sponsored by

Professor of Tumor Pathology & Head of the Molecular Pathology Laboratory,
University Medical Center Essen, Germany

Staff Scientist,
Scientific Affairs,
Agena Bioscience GmbH

This webinar discusses advances in detecting MET and NTRK variants in tumor samples, which holds significant potential for diagnostic and research applications.  

MET oncogenic variants leading to diverse exon 14 splicing alterations are emerging as a new predictive biomarker that would be sensitizing to MET-targeted tyrosine kinase inhibitors. The splice site DNA somatic variants result in RNA splicing-based skipping of MET exon 14, which supports targeted therapies.

Fusions in the NTRK1, NTRK2 and NTRK3 genes, which encode neurotrophin receptors TRKA, TRKB and TRKC, result in overexpressed kinase function, leading to oncogenesis in a wide variety of adult and pediatric solid tumors. Novel compounds have recently been developed that selectively inhibit TRK fusion proteins. As these alterations affect multiple histologies, detecting the presence of fusions across these genes greatly advances clinical cancer research.

In this webinar, Prof. Hans-Ulrich Schildhaus presents results from a study using a targeted, MALDI-TOF based method for the detection of MET exon 14 alterations and fusions across NTRK1, NTRK2 and NTRK3, and its applicability for routine screening.

Laboratory Diagnostician,
Laboratory of Medical Genetics, St. Elizabeth Oncology Institute

This webinar discusses a cancer center's hands-on experience with a targeted approach for clinical cases of hereditary cancers.

Next-generation sequencing (NGS) in routine genetic diagnostic practices offers a rapid and cost-effective analysis for identifying pathogenic variants associated with hereditary cancer syndromes. To date, the Laboratory of Medical Genetics at St. Elizabeth Oncology Institute in Bratislava has analyzed more than 2,500 families with suspected hereditary cancer syndromes using the NGS technology.

The adoption of NGS not only allows for the simultaneous analysis of multiple genes but also accelerates the turnaround time for high sample volumes. However multi-gene NGS applications also present new challenges, such as the management of big data, the accurate detection and classification of genetic variants.

In this webinar, Dr. Jana Ziburova, Laboratory Diagnostician at St. Elizabeth Oncology Institute, shares her experience and describe:

  • An approach to detect hereditary cancers using the solution from Sophia Genetics
  • Benefits and challenges of using multi-gene NGS in complex clinical cases
  • How to identify pathogenic variants in unexpected genes not directly related to the disease

Associate Laboratory Director,
Versiti (formerly Blood Center of Wisconsin)

This webinar tells the story of Versiti's journey in transforming genetic testing from a manual to a digitized process. It includes detail on how the organization succeeded, pain points along the way, a novel approach to variant assessment, and future plans for the program.

Versiti (formerly Blood Center of Wisconsin) specializes in a wide range of services, including esoteric diagnostic testing, such as immunology, hematology, oncology, and serology.

In this session, Dr. Valerie Trapp-Stamborski covers:

  • Bringing genetic testing onto a technical platform for improved efficiency, analysis, and reporting.
  • The anatomy of a novel variant assessment tool that is used to classify and assess variants of uncertain significance.
  • The organization's efforts around integrated reporting for improved diagnostic insights.

Research Specialist,
Perelman School of Medicine at the University of Pennsylvania

Assistant Professor, Department of Genetics,
University of Pennsylvania

Senior Product Manager
Swift Biosciences

This webinar illustrates how single-cell methylation sequencing can be applied to gain significant insight into epigenetic heterogeneity in disease states, advancing cancer research discoveries. 

Our speaker, Jennifer Flournoy of Perelman School of Medicine at the University of Pennsylvania, discusses a single-cell methylation approach from Swift Bioscience that her team is using to study pediatric leukemia and gliomas.

Swift's Accel-NGS Adaptase Module enables construction of next-generation sequencing libraries from bisulfite-converted, single-stranded DNA from single cells. This module is optimized to maximize the recovery of DNA containing uracil residues and low concentrations of AT-rich template. The resultant libraries consistently exhibit superior complexity with reduced composition bias to provide a more faithful representation of the methylome. This approach has demonstrated greater than 2-fold increase in read mapping rate as compared to other methods, significantly improving the data output per run while reducing the sample sequencing cost.

In this webinar, Jennifer Flournoy and her colleague Dr. Hao Wu details how they are using this single-cell methylation method to contribute to the generation of multi-omic human tumor atlases and to inform therapeutic approaches for pediatric cancers.

Head of Medical Genomics,
Glasgow Precision Oncology Laboratory

Genomics is a key element in the potential of precision medicine to transform oncology. Hybrid-capture based targeted next-gen sequencing (NGS) represents a particularly promising option for cancer genomic testing, as it enables the profiling of all cancer-relevant events with the advantage of being compatible with formalin-fixed, paraffin-embedded (FFPE) tissue.

The Glasgow Precision Oncology Laboratory (GPOL) has defined the content required to capture the full range of clinically and biologically important genomic events that characterize solid tumors and turned these into a range of SureSelect cancer NGS assays, the first of which, dubbed Cancer Core, will be presented during this webinar.

In this webinar, Dr. Cooke, head of Medical Genomics of the GPOL shares:

  • How the GPOL has built affordable, fit-for-purpose, cancer NGS solutions using the SureSelect target enrichment platform, specifically to address the challenges of real-world oncology.
  • How the GPOL is implementing broad genomic testing as part of its mission to accelerate the translation of novel cancer therapeutics from discovery to clinical applications.

 For Research Use Only. Not for use in diagnostic procedures.

Pathology Department and Cancer Research Division,
Peter MacCallum Cancer Centre, Melbourne, Australia

This webinar will provide an overview of how a pathology laboratory validated a 77-gene next-generation sequencing-based liquid biopsy assay.

Liquid biopsy provides a minimally invasive alternative to tissue biopsy for diagnosis, treatment selection, and monitoring of solid cancers. Liquid biopsy, especially the detection of molecular biomarkers from circulating tumor DNA (ctDNA), has evolved from single biomarker to multi-biomarker measurement with the application of NGS technologies, an advance that promises to improve clinical sensitivity — particularly for the detection of disease relapse and treatment resistance.

To date, the ctDNA-NGS space has been dominated by well resourced commercial providers offering accredited services based on laboratory developed tests. This webinar will describe how the Pathology Department at Australia's Peter MacCallum Cancer Centre is looking to bring this capability in house. Peter Mac's Andrew Fellowes will describe the analytical performance of the Avenio ctDNA Expanded Kit* in a cancer hospital pathology laboratory.

The Avenio ctDNA Expanded Kit* is a commercially available research use only kit based on Cancer Personalized Profiling by Deep Sequencing (CAPP-Seq) that promises to accelerate the uptake of liquid biopsy for clinical research of solid cancers in the clinical laboratory setting.

*For Research Use only, not for use in diagnostic procedures.

Wed
Sep
25
12:00 pm2019
Sponsored by
HalioDx

Biomarker Evaluation for CAR T-Cell Therapy: Impact on Drug Development

Genome Webinar

Vice President of Translational Medicine
Kite, a Gilead Company

CAR T-cell therapy is an innovative form of immunotherapy that is finding increasing use for the management of blood cancers. While this approach has demonstrated impressive results for some patients, there are still challenges such as toxicity and difficulty in predicting which patients will respond.

The immune microenvironment is one of the factors that influences clinical outcomes, so identifying immune biomarkers is critical to maximizing the benefits of CAR T-cell therapy.

During this webinar, Adrian Bot, Vice President of Translational Medicine at Kite, a Gilead Company, explains how biomarker evaluation for CAR T-cell therapy can elucidate the mechanism of action and impact drug development.

This webinar also includes a brief overview of HalioDx's Immunogram platform and how it can provide meaningful and actionable data through biomarker testing.

Viewers of this webinar will learn:

  • How CAR T-cell therapy fights cancer cells and the associated mechanism of action.
  • Why evaluating immune biomarkers in CAR T-cells is crucial.
  • How biomarker evaluation can impact immunotherapy drug development.
Sponsored by

Postdoctoral Research Fellow,
Cancer Research UK Edinburgh Center,
MRC Institute of Genetics and Molecular Medicine

This webinar discusses a study that compared nine different transcriptomic analysis technologies with matched fresh frozen (FF) and formalin-fixed paraffin-embedded (FFPE) cancer tissues.

Cost and tissue availability normally preclude processing samples across multiple technologies, making it difficult to directly evaluate performance, reliability, and to what extent gene expression data from different platforms can be compared or integrated. In order to explore the feasibility of integrating gene expression data from different platforms, Dr. Arran K. Turnbull of the Cancer Research UK Edinburgh Center and colleagues explored nine technologies, which varied in resolution, cost, and RNA requirements.

The study used sequential tumor biopsies from 11 postmenopausal women with estrogen receptor positive breast cancer treated with three months of neoadjuvant anti-estrogen therapy. Half of each sample was snap frozen in liquid nitrogen and half was FFPE.

Transcriptomic analyses were performed using the Illumina Beadarray, Affymetrix U133A, Affymetrix Clariom S, NanoString nCounter, AmpliSeq Transcriptome, Lexogen QuantSeq and IonXpress RNAseq, Tempo-Seq BioSpyder, and Qiagen UPX 3’.

 Dr. Turnbull details the study’s findings, which include: 

  • Robust gene expression profiles can be reliably generated from FFPE tissues and are comparable to those derived from FF tissue using established transcriptomic approaches.
  • A range of new technologies are available for the study of FFPE tissues; these vary in cost, resolution, and RNA requirements to fit the user’s needs.
  • Gene expression data from biologically similar studies, generated using different technologies, can be reliably integrated for robust meta-analysis, subject to appropriate batch correction analysis.

Postdoctoral Researcher,
Max Delbrück Center for Molecular Medicine 

In this webinar, Ngoc-Tung Tran, Postdoctoral Researcher at the Max Delbrück Center for Molecular Medicine, will provide a general introduction of the CRISPR/Cas9 system. He will summarize the current approaches to enhanced homology-directed repair for precise gene editing. Regarding gene therapy applications, he will point out the differences between precise gene editing by CRISPR/Cas9 and gene delivery by viruses. He will also discuss the potential limitations of CRISPR/Cas9 for clinical applications as well as the current status of solving these limitations.

The webinar will include an example of gene correction using CRISPR/Cas9 in his lab. Specifically, he will discuss ELANE mutation correction in patient-derived hematopoietic stem cells, and the potential of this approach as a potential gene therapy for severe congenital neutropenia.

Director, Molecular Diagnostics Lab; Professor Pathology; Chair of Molecular Diagnostics Division
Virginia Commonwealth University Health System

This webinar discusses a next-generation sequencing approach for detecting genomic mutations in hematologic maglignancies.

Join Dr. Andrea Ferreira-Gonzalez, Professor of Pathology and Chair of Molecular Diagnostics Division at Virginia Commonwealth University Health System, to learn about the role of genomic testing in hematology.

Dr. Ferreira-Gonzalez introduces the different hematological disease states, their biology, and driver mutations. She provides an overview and background about the role these mutations play in blood cancers and the steps taken for the validation and implementation of a clinical assay for hematological malignancies using RNA and DNA as detection methods for NGS.

Head, Division of Genetics Department of Lab Medicine and Pathology,
Saint John Regional Hospital

This webinar provides a first-hand look at how a molecular laboratory validated and implemented a targeted next-generation sequencing-based myeloid assay to expedite the assessment of myeloid malignancies and assist in the understanding of myeloid cancers.

The most recent version of the World Health Organization classification system for myeloid neoplasms and acute leukemia, published in 2016, added a number of important biomarkers and genetic alterations for the assessment of myeloid malignancies. As the list of relevant molecular markers continues to grow and new targeted therapies are approved, traditional, single-gene approaches for analyzing myeloid malignancies have become laborious and time consuming. Next-generation sequencing has emerged as the optimal solution by enabling comprehensive assessment of all relevant molecular markers in a single NGS run.

In this webinar, Dr. Nancy Carson, Head of the Division of Genetics at the Saint John Regional Hospital, discusses her team’s experiences as one of the first labs in Canada to implement an NGS-based myeloid assay.

Dr. Carson discusses:

  • Unique considerations and applications of NGS in myeloid malignancies
  • Overview of her experience with with analytical validation and implementation of the assay
  • Impact of the implementation of the assay to date through case studies
  • Future directions

Professor Genetics KU Leuven, Group Leader VIB, Leuven, Belgium

Postdoctoral Researcher, VIB-KU Leuven, Belgium

This webinar outlines a project that performs large-scale and integrative single-cell genome and transcriptome profiling of pediatric acute lymphoblastic leukemia (ALL) cases at diagnosis, during drug treatment, and in case of relapse.

ALL is the most common cancer in children and shows extensive genetic intra-tumoral heterogeneity. This heterogeneity may be the underlying reason for an incomplete response to treatment and for the development of relapse.

Data from this study provides information about the sensitivity of each leukemia clone to therapy and about how relapse can develop. Moreover, the results point toward the feasibility to detect minor clinically relevant leukemia clones at diagnosis or during early days of treatment in ALL.

The main focus of this webinar is:

  • Introduction of the Tapestri Platform from Mission Bio for targeted single-cell DNA sequencing
  • Presentation of a novel custom panel covering the 300 most mutated genomic regions in ALL
  • Insights into the clonal architecture of pediatric T-ALL, lessons learned from the first 16 samples processed with this custom ALL panel
Sponsored by
Dr. Yaolin Zhou discusses Quality Improvement Model to Support Molecular Testing of Oncology Patients

Director of Molecular Pathology,
University of Oklahoma Health Sciences Center (OUHSC)

This webinar discusses how the Molecular Pathology Laboratory at the University of Oklahoma (OUMP) is using a new quality improvement model to support molecular testing of oncology patients. 

The oncology landscape is rapidly evolving due to new biomarker discoveries and targeted treatments. Biomarker testing is ideally performed in on-site molecular diagnostic laboratories to facilitate local communication and promote multidisciplinary collaboration.

However, assay implementation and incorporation is complex and full of potential pitfalls. Due to the costs and challenges associated with offering new molecular tests, labs need to take additional steps to ensure that the healthcare provided is effective, efficient, patient-centered, safe, and timely.

In this webinar, OUMP's Yaolin Zhou discusses how her lab approaches molecular testing as a quality improvement initiative. She presents the EPIDEM model of quality improvement, which stands for Exploration, Promotion, Implementation, Documentation, Evaluation, and Modification.

Dr. Zhou reviews specific applications of the EPIDEM model to improve molecular testing of leukemia, breast cancer, and melanoma patients and will also share OUMP’s approach to next generation sequencing using Qiagen's GeneReader NGS System.

Associate Director, Laboratory for Molecular Pediatric Pathology; Staff Pathologist, Boston Children's Hospital; Instructor of Pathology, Harvard Medical School

This webinar discusses background and clinical genomics of NTRK fusion detection in cancer. NTRK fusions are the focus of new therapeutic options, but clonal and subclonal lesions are notoriously difficult to detect. This webinar provides an overview and background about the increased role of these fusions, and latest trends in diagnosis, prognosis, and treatment, as well as a research case study on detection.

Join Dr. Alanna Church of the Laboratory for Molecular Pediatric Pathology and Staff Pathologist at Boston Children's Hospital to learn more about the increasing role of NTRK fusions:

  •  Overview and background of fusion mutations, specifically NTRK 1, 2, and 3
  •  Frequency overview and specificity needed for detection
  •  Overview of research case of utilizing NGS technology in detection.

R&D Manager, ID-Solutions

VP of Commercial Operations, Stilla Technologies

This webinar will outline the entire liquid biopsy workflow from cell-free DNA isolation to mutation detection by Crystal Digital PCR with the Naica System from Stilla Technologies.

Our speakers will focus on detecting EGFR, BRAF, NRAS, and KRAS mutations as well as pediatric and adult cerebral tumor classification panels.

Attendees of this webinar will:

  • Understand the liquid biopsy process for EGFR, BRAF, NRAS, and KRAS mutations;
  • Learn about the benefits of the Crystal Digital PCR platform in combination with research-use-only kits;
  • Hear why digital PCR is a particularly useful technique for the detection of mutations, therapeutic monitoring, and resistance appearance;
  • Learn about the different steps of the liquid biopsy workflow, from DNA isolation to DNA quantification and qualification and DNA genotyping, with dPCR multiplex kits

Senior Specialist Biomedical Scientist, Frontier Pathology

Specialist Biomedical Scientist, Frontier Pathology

Specialist Biomedical Scientist, Frontier Pathology

This webinar will provide a first-hand look at how a hematology/oncology lab in the UK set up and validated three molecular assays for routine in-house use.

Speakers from the Royal Sussex County Hospital (RSCH) laboratory, operated under the Frontier Pathology NHS Partnership, will share their experience implementing two assays for suspected BCR-ABL1-negative myeloproliferative neoplasms.

The RSCH lab has spent the last several years repatriating historical send-away hemato-oncology assays for JAK2 V617F and CALR exon 9. During this webinar, RSCH scientists Munyoro Guvamatanga, Anna Tarasewicz, and Rebecca Lough will share their experiences bringing these assays in-house.

The JAK2 V617F mutation assay was the first to be repatriated in 2015 and is performed using the CE-IVD marked ipsogen JAK2 RGQ PCR kit. More recently, the lab began detecting CALR exon 9 mutations using the CE-IVD marked ipsogen CALR RGQ PCR kit. The assays are performed using gDNA extracted from whole blood samples and subsequent real-time qPCR on the QIAGEN Rotor Gene Q MDx 5Plex HRM platform.

This webinar will describe the experiences and challenges associated with the setup and validation/verification of the assays in the RSCH laboratory.