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White Papers and Videos

A New Scalable and Automatable Method for the Extraction of cfDNA

White Paper

Liquid biopsies represent a promising area of cancer testing as taking blood is less invasive than tumor biopsies. The cell-free DNA (cfDNA) present in the blood includes DNA derived from cancer cells and cancer biomarkers can be detected in the extracted cfDNA. Whole blood also contains genomic DNA and can be removed by centrifugation, resulting in plasma.

cfDNA is present in very small amounts in blood or plasma, and thus larger amounts of plasma are required for many applications. Larger extractions are more challenging to automate, as they require additional pipetting steps.

This poster from Beckman Coulter Life Sciences presents a novel cfDNA extraction kit, discusses its optimization, and demonstrates automation on a KingFisher Duo, showing that this kit can be used for NGS and can produce results comparable to other commercial kits.

Cell-Free DNA Extraction Efficiency Affects NGS Data

White Paper

This technical note from Beckman Coulter Life Sciences presents data from a study in which EGFR L858R standards with varying allele frequencies were spiked into plasma and isolated using either Apostle MiniMax High-Efficiency cfDNA Isolation or another extraction product, showing extraction efficiency to affect NGS results.

A Workflow for Medium-Throughput Isolation of cfDNA From Plasma Samples Using Apostle Minimax on the KingFisher Technology

White Paper

Due to its small size and low quantity, the main challenge for cell-free DNA analysis is to get enough cfDNA for sequencing. This results in a need to extract from larger volumes of bodily fluid, yet higher input volumes can be more difficult to manage for high-throughput sample processing.

This application note from Beckman Coulter Life Sciences demonstrates the use of the Apostle MiniMax High-Efficiency cfDNA Isolation Kit in conjunction with the KingFisher Duo Prime automated protein purification system to mitigate some of the challenges of processing large-volume samples for cell-free DNA sequencing.

Correlation Between Mutations Found in FFPE Tumor Tissue and Paired cfDNA Samples

White Paper

As a non-invasive way to detect disease, cfDNA is extracted from blood; however, there is some concern that cfDNA does not contain the same biomarkers as tumor tissue. Tumor tissue is typically removed and stored as formalin-fixed, paraffin-embedded tissue, a process that preserves the morphological structures well but chemically modifies and degrades the nucleic acids. Despite the difficulties, FFPE tissue is often used to look for cancer-associated mutations; however, it does not always correlate with the mutations seen in cfDNA.

This poster from Beckman Coulter Life Sciences presents a comparison of matched FFPE and plasma samples to determine how many mutations are seen in both tissues and where the mutational mismatches appear in the chromosome, finding that chromosomal regions have different mismatch rates and giving clues about the best chromosomal locations for biomarkers.

Cell-Free DNA Extraction from Plasma for Liquid Biopsy

White Paper

This white paper from Beckman Coulter Life Sciences presents data generated by the Apostle MiniMax High-Efficiency cfDNA Isolation Kit, a cell-free DNA (cfDNA) isolation reagent kit built on magnetic bead-based technology demonstrated to purify cfDNA from human plasma in both manual and automated workflows.

Comparative Analysis of Cell-Free DNA Extraction Efficiency from Plasma

White Paper

With recent advancements in nucleic acid-based molecular technologies, applications for precision medicine in clinical research have grown exponentially. In particular, analysis of cell-free DNA (cfDNA) for cancer detection, prognosis, and monitoring has significantly improved patients’ clinical management and outcomes.

The primary challenge with cfDNA-based liquid biopsy techniques is that cfDNA is typically found at very low concentrations in body fluids, with a range of 1 to 50 ng cfDNA from 1 mL plasma. Of the total cfDNA found in plasma, fragments originating specifically from tumors (ctDNA) can represent as low as 0.01 percent. Thus, efficient techniques for cfDNA extraction are essential for ctDNA detection in precision medicine. Two of the most commonly used cfDNA extraction techniques are spin column-based and magnetic bead-based purification.

This technical note from Beckman Coulter Life Sciences describes a study comparing a spin column-based cfDNA purification kit with a magnetic bead-based purification kit, finding the bead-based kit to significantly outperform the column-based kit and highlighting the importance of selecting an efficient cfDNA extraction method for liquid biopsy applications.

Study Shows Clinical Decision Support Software Exceeds Consistency Among Variant Scientists

White Paper

Numerous clinical decision support systems and knowledgebases have been developed to assist variant scientists and laboratory directors with the task of variant classification. These private and commercially available systems utilize varying degrees of software automation and manually curated literature to provide variant assessment and therapy matching for clinicians. The body of literature that must be accessed to deliver accurate variant interpretation is vast and there is debate in the field as to the most accurate and efficient approach.

This white paper from Qiagen describes a study demonstrating that clinical decision support tools can help to reliably streamline and standardize somatic variant interpretation and address the high degree of variability among experts in somatic clinical interpretation.

Unlock Greater Insights with Multimodal and Multiomics Data Integration and Analysis

White Paper

The new era of personalized health relies on data to guide more personalized patient treatments, therapeutics, diagnostics, and patient care. Across clinical and research disciplines, leading healthcare and life sciences organizations are combining varying data modalities to increase the accuracy of diagnoses, reduce turnaround times, and ultimately improve patient outcomes.

This white paper from Amazon Web Services presents case studies in which companies, hospitals, and organizations partnered with AWS for Health to unify analysis of various forms of medical and omics data and help researchers and clinicians generate new insights and offer more personalized care.

Rapid-Turnaround Oncomine Precision Assay Enables Global Harmonization for Clinical Trial Testing

White Paper

Genetic variation in neoplasia can take the form of single or multiple nucleotide variants, gene fusions, amplifications, and/or splice variations. This heterogeneity can pose a challenge in comprehensive profiling to guide therapeutic decision making. The Oncomine Precision Assay on the Genexus Integrated Sequencer is an amplicon-based automated NGS assay that enables the detection of all these genomic variant classes, spanning 50 genes, focused on those with the greatest clinical relevance and targets of many ongoing trials. The complete workflow, from library prep to final report generation, can be accomplished in less than 30 hours for up to 32 formalin-fixed paraffin-embedded samples. OPAGX uses AmpliSeq HD technology, utilizing unique molecular tags for sequencing error correction and increased sensitivity of variant detection.

This poster from Q2 Solutions describes a study in which the Oncomine Precision Assay on the Genexus Integrated Sequencer was characterized at a Durham, NC-based site and then transferred to and validated by a genomics laboratory in Beijing, enabling global harmonization of clinical trial testing.

Evaluation and Reproducibility Study Using NanoString GeoMx Immune Pathway Panel

White Paper

The rapidly advancing field of spatial biology has led to the development of new platforms that provide measurements of gene and protein expression and their precise localization to defined tissue regions. The NanoString GeoMx DSP (digital spatial profiling) platform enables spatial profiling using formalin-fixed, paraffin-embedded tumor biopsy samples with a read out using either the nCounter or next-generation sequencing.

This poster from Q2 Solutions presents an examination of both healthy tonsil sections and a well-characterized breast carcinoma biopsy with spatial analysis in a proof-of-concept study examining variability across days, patient biopsies, and sample stability using the NanoString GeoMx Immune Pathway Panel.

Global Site Reproducibility of Characterizing Biomarkers in cfTNA from Liquid Biopsy Samples Using the Targeted Oncomine Precision Assay

White Paper

High-complexity NGS workflows impact the ability to achieve consistent inter-laboratory performance. Often, interlaboratory performance is highly dependent on operator proficiency and institutional systems designed to minimize the variability of testing performance.

This poster from Q2 Solutions describes the analytical performance and reproducibility of an automated next-generation sequencing pan-cancer DNA and RNA assay at two global laboratory sites located in Durham, NC and Beijing, demonstrating interlaboratory accuracy and reproducibility enabling global implementation and harmonization for clinical testing.

PINC AI Applied Sciences (PAS): Advancing Health Equity Roadmap

White Paper

This white paper from PINC AI discusses the causes of health inequities and how equity may be advanced through innovative and collaborative solutions that address the underlying systemic issues that contribute to inequities, and it describes a case study in which PINC AI collaborated with Henry Ford Health to develop solutions.

Reference Materials for Analysis of BRCA1 and BRCA2 Gene Variants with Expansion to Large Genomic Rearrangements (LGRs) to Support Therapy Selection in Breast and Ovarian Cancer Patients

White Paper

Mutations in BRCA1 and BRCA2 tumor-suppressor genes account for a significant portion of hereditary breast and ovarian cancer cases. Among them, large genomic rearrangements (LGRs) involving a loss or gain of partial complete BRCA exon(s) are responsible for up to 27 percent of all BRCA disease-causing mutations identified. However, these LGRs are frequently missed using both PCR-based methods and targeted NGS assays.

Given the difficulty in detecting LGRs, there is a need for a comprehensive BRCA1/2 testing algorithm including reference materials that incorporate pathogenic BRCA1/2 LGRs to support NGS assays that identify these mutations at both germline and somatic levels. Clinical studies have demonstrated that pathogenic BRCA1/2 mutations sensitized patients to platinum-based chemotherapy and PARP inhibitors. Current guidelines increasingly recommend BRCA testing in management and therapy selection. Accurate detection of BRCA1 or BRCA2 pathogenic variants is therefore critical in breast and ovarian cancer therapy as well as clinical management of disease including patients who are eligible for new PARP inhibitors.

This poster from LGC SeraCare describes the development and validation of reference material to support BRCA1 and BRCA2 gene testing, the first to include 10 LGRs, which will be useful in evaluating the ability to detect challenging LGRs and support PARP inhibitor treatment of patients with breast and ovarian cancers.

Reference Materials for the Analysis of Methylation in Circulating Cell-Free DNA

White Paper

Epigenetic modifications such as methylation influence cellular differentiation and gene expression. Liquid biopsies are starting to screen for cancer-derived DNA by looking for unexpected epigenetic modifications in circulating cell-free DNA (ccfDNA) that could also be used to assign a tissue of origin to the cancer to direct confirmatory diagnostic procedures. However, because of the low abundance of ccfDNA, obtaining sufficient amounts for assay development, validation, and proficiency testing are difficult. Furthermore, methods of analyzing the epigenetic modifications, such as bisulfite conversion, can damage a significant fraction of the input material; but, failing to carry them out to completion can result in an overestimation of methylation. This makes method optimization very important.

This poster from LGC SeraCare describes the creation and validation of circulating cell-free DNA reference materials with defined amounts of methylation to address the challenges associated with assessing the methylation of circulating cell-free DNA.

Development of Seraseq FFPE Homologous Recombination Deficiency Reference Materials for NGS Assay Validation

White Paper

Homologous recombination deficiency (HRD) arises due to a defect in DNA repair and serves as an important therapeutic biomarker. NGS assays that measure HRD status via genomic instability are used to stratify ovarian and breast cancer patients and determine eligibility for clinical trials as well as PARP inhibitor and platinum-based therapies. Testing is performed on FFPE biopsied tissue, and, despite the challenges of this sample type and the complexity of analysis, there are currently no commercially available HRD reference materials that enable standardization between assays.

This poster from LGC SeraCare describes the development and validation of reference materials to aid in developing and validating NGS-based HRD assays and for quality control monitoring of genomic instability scores.