A New Scalable and Automatable Method for the Extraction of cfDNA
Liquid biopsies represent a promising area of cancer testing as taking blood is less invasive than tumor biopsies. The cell-free DNA (cfDNA) present in the blood includes DNA derived from cancer cells and cancer biomarkers can be detected in the extracted cfDNA. Whole blood also contains genomic DNA and can be removed by centrifugation, resulting in plasma.
cfDNA is present in very small amounts in blood or plasma, and thus larger amounts of plasma are required for many applications. Larger extractions are more challenging to automate, as they require additional pipetting steps.
This poster from Beckman Coulter Life Sciences presents a novel cfDNA extraction kit, discusses its optimization, and demonstrates automation on a KingFisher Duo, showing that this kit can be used for NGS and can produce results comparable to other commercial kits.